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Image Search Results
Journal: Clinical cancer research : an official journal of the American Association for Cancer Research
Article Title: Abemaciclib is Active in Preclinical Models of Ewing’s Sarcoma via Multi-pronged Regulation of Cell Cycle, DNA Methylation, and Interferon Pathway Signaling
doi: 10.1158/1078-0432.CCR-18-1256
Figure Lengend Snippet: Mice bearing RD-ES xenografts were treated orally with 50 mg/kg abemaciclib (■) or vehicle (●) daily. A, Tumor growth over time. Dotted lines indicate dosing interval. Mean ± SEM is displayed, n = 3/group. B and C, Xenografts were harvested after 10 days of treatment. B, IHC analysis for cell death (TUNEL), cell proliferation (Ki67), and cell number (DNA content). Bar graphs under each set of representative images quantify staining for each endpoint, n = 8 fields/group. One-way ANOVA with Dunnett’s comparison. C, Analysis of protein expression by immunoblotting. Protein levels were quantitated using the Chemidoc XRS software (BioRad). Reported values are normalized to G PDH (n = 8/group). Error bars: SEM. Paired Student’s t-test; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.
Article Snippet: Protein levels were quantitated using the
Techniques: TUNEL Assay, Staining, Comparison, Expressing, Western Blot, Software